molecular phylogeny of malaysian pangasiid based on cytochrome c oxidase i (coi) dna sequences

the molecular phylogenetic trees of 42 representative samples of malaysian pangasiid were constructed using cytochrome c oxidase subunit i (coi). the topologies resulting from molecular analyses, maximum parsimony, maximum likelihood, and bayesian inference, revealed the paraphyly of pangasius genus and monophyly of p. nasutus, p. bocourti, p. micronemus, and p. hypopthalmus with high support values. the results clearly showed that coi gene can be used to isolate the native species of malaysian pangasiids, including p. nasutus, p. micronemus, p. bocourti and h. waandersii from p. hypopthalmus that has been introduced from thailand.